LIPID PROFILE AND PLATELET AGGREGATION OF ETHANOLIC SEED EXTRACT OF AVOCADO (PERSEA AMERICANA MILL.) IN HYPERLIPIDEMIC MALE WISTAR RAT
Objective: The objective of this research was to investigate the effect of ethanol seed extract of avocado (ESEA) (Persea americana Mill.) on lipid
profile and platelet aggregation in hyperlipidemic male Wistar rat.
Methods: The avocado seed was extracted using 70% ethanol by the reflux method. The induction method was conducted by giving high cholesterol
intake and oral administration of pure cholesterol, cholic acid, and propylthiouracil. Antiplatelet aggregation parameters were measured by observing
of bleeding time (BT), coagulation time (CT), and antiplatelet aggregation activity using adenosine 5'-diphosphate (ADP) as aggregation inductor.
Lipid profile such as total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL), and low-density lipoprotein (LDL), as well as the
parameters of platelet aggregation were measured before the induction, the end of induction and the end of therapy.
Results: ESEA 10 mg/kg bw showed decreasing in TC and LDL levels which was significantly different compare to control group (p<0.05) while ESEA
20 and 40 mg/kg bw gave decreasing in TG. All of doses of extract showed no increasing in HDL levels. In antiplatelet aggregation effect test, ESEA
10 mg/kg bw had the ability to extend BT. ESEA 20 and 40 mg/kg bw had the ability to extend the CT. All of doses of extract showed significantly
different (p<0.05) on the measurement of aggregation activity by decreasing in plasma absorbance.
Conclusion: The result suggested lipid lowering and anti-aggregation potential of ESEA, which serves as a new potential herbal product and good for
cardiovascular disease treatment because it can reduce both risk factors causing the disease.
Keywords: Cholesterol, Platelet aggregation, Avocado, Persea americana Mill., Seed.
Jahnavi N, Naidu PP, Rao K, Karunasree CP. Antihyperlipidemic effect
of Asparagus gonnoclados baker against cholesterol diet induced
hyperlipidemia in rats. Asian J Pharm Clin Res 2014;7(2):212-5.
van der Stoep M, Korporaal SJ, Van Eck M. High-density lipoprotein
as a modulator of platelet and coagulation responses. Cardiovasc Res
Brenner GM, Stevens CW. Anticoagulant, antiplatelet and fibrinolytics
drugs. In Pharmacology. 2
ed. Amsterdam: Saunders Elsevier; 2006.
Asaolu MF, Asaolu SS, Oyeyemi AO, Aluko BT. Hypolipemic effects of
methanolic extract of Persea americana seeds in hypercholesterolemic
rats. J Med Sci 2010;1(14):126-8.
Sodipo OA, Akiniyi JA. Studies on certain characteristics of extracts
from bark of Pausinystalia macroceras (K. schum). Pierre Exbeille.
Glob J Pure Appl Sci 2000;6:83-7.
Arukwe U, Amadi BA, Duru MK, Agomuo EN, Adindu EA, Odika PC,
et al. Chemical compositon of Persea americana leaf, fruit and seed.
Int J Res Rev Appl Sci 2012;11(2):346-9.
Asian J Pharm Clin Res, Vol 9, Issue 1, 2016, 143-147
Soemardji et al.
Duke J. Handbook of Biological Active Phytochemicals and their
Activities. Boca Raton, FL: CRC Press; 1992. p. 99-131.
Pahua-Ramos ME, Ortiz-Moreno A, Chamorro-Cevallos G,
HernÃ¡ndez-Navarro MD, GarduÃ±o-Siciliano L, NecoecheaMondragÃ³n
of avocado (Persea
Hum Nutr 2012;67(1):10-6.
Permatasari A. The effect of simvastatin, aspirin, their combination
and bungur ethanolic extract (Lagerstroemia speciose (L.) Pers.) in
initiation step of atheroma plaque. School of Pharmacy-Bandung
Institute of Technology; 2014. p. 26-7.
Friedewald WT, Levy RI, Fredrickson DS. Estimation of the
concentration of low-density lipoprotein cholesterol in plasma, without
use of the preparative ultracentrifuge. Clin Chem 1972;18(6):499-502.
Venkataraman S, Meera R, Ramchandran V, Devi P, Aruna A,
Parameswari SP, et al. Antioxidant and anticoagulant activity of novel
n-substituted 4 methyl 5,7 dihydroxylcoumarin and its ester derivatives.
Int J Pharm Rev Res 2004;4(1):25-32.
Sukandar EY, Sigit JI, Fitriyani N. Antiplatelet aggregation of garlic
(Allium sativumL.) water extract, turmeric (Curcuma domestica Val.)
ethanolic extract and its combination to Swiss Webster male mice.
Indones J Pharm 2008;19(1):1-11.
Vogel HG. Drug Discovery and Evaluation, Pharmacological Assay.
ed. New York: Springer-Verlag Berlin Heidelberg; 2008.
Yang W, Jun WD, Zhang R, Deng Q, Ding H, Tian Y, et al. Effects
of propylthiouracil-induced hypothyroidism on nonalcoholic fatty
liver disease in rats fed a high-fat and high-cholesterol diet. Pak Vet J
Barth CA. Regulation and interactions of cholesterol, bile salt, and
lipoprotein synthesis in liver. Klin Wochenschr 1984;163-70.
Radcliffe JD, Liebsch KS. Dietary induction of hypercholesterolemia
and atherosclerosis in Japanese quail of strain SEA. J Nutr
Nwanjo HU. Efficacy of aqueous leaf extracts of Vernonia amygdalinaon
plasma lipoprotein and oxidative status in diabetic rats models. Niger J
Physiol Sci 2005;20:39-42.
Gerd A, Gotto AM. HDL cholesterol and protective factors in
atherosclerosis. Circulation 2004;109:8-14.
Villa J, Pratley RE. Ezetimibe/simvastatin or atorvastatin for the
treatment of hypercholesterolemia in patients with the metabolic
syndrome: The VYMET study. Curr Diab Rep 2010;10(3):173-5.
McCormack T, Harvey P, Gaunt R, Allgar V, Chipperfield R, Robinson P.
Incremental cholesterol reduction with ezetimibe/simvastatin,
atorvastatin and rosuvastatin in UK General Practice (IN-PRACTICE):
Randomised controlled trial of achievement of Joint British Societies
(JBS-2) cholesterol targets. Int J Clin Pract 2010;64(8):1052-61.
Goldberg IJ. Diabetic dyslipidemia: Causes and consequences. J Clin
Endocrinol Metab 2001;86:965-71.
Prociuk MA, Edel AL, Richard MN, Gavel NT, Ander BP,
Dupasquier CM, et al. Cholesterol-induced stimulation of platelet
aggregation is prevented by a hempseed-enriched diet. Can J Physiol
How to Cite
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.