SUBCHRONIC TOXICITY STUDY OF CORN SILK (ZEA MAYS L.) IN COMBINATION WITH BINAHONG (ANREDERA CORDIFOLIA (TEN.) STEENIS LEAVES ON WISTAR RATS
Objective: Nowadays, modern medication has been shifting into natural-based medication. These phenomena lead to the elevation of the use of
traditional medicine. Similar to modern medicine, nature-based medicine should meet the standard which is including safety, efficacy, and quality
aspects. The medicine must be safe during the consumption period and scientifically proven through preclinical toxicity test. It can be obtained
through acute and subchronic toxicity test. Besides, it should have abilities to treat certain diseases and ease of accessing such medicines should be
guaranteed particularly for patients. From our previous study, a combination of ethanolic extract of corn silk (CS) and binahong leaves (BL) showed
activity in ameliorating kidneys function on renal failure ratâ€™s model. Therefore, to obtain standardized traditional medicine, a study on safety must
Methods: A 90-day oral toxicity study using Wistar rats was conducted to evaluate subchronic toxicology. The combination of extracts were given in
three different levels daily for 90 days and a 120 days toxicity test was carried out for satellite groups.
Results: Administration of extracts in combination to rats for 90 days did not alter behavioral, motoric activities, urine, hematological, chemical,
and histopathological parameters, and it was suggested a no-observed adverse-effect level of CS 450 mg/kg and BL 600 mg/kg in combination. Even
though the number of white blood cells in all treated groups arose compared to the control group, but the values were still in the normal range.
Conclusion: These results indicate that the combination of ethanolic extract of CS and BL can be generally meet safety requirement as traditional
Keywords: Corn silk, Zea mays L., Binahong leaves, Anredera cordifolia (Ten.) Steenis, Subchronic toxicity test.
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