POTENTIAL OF CELLULASE OF PENICILLIUM VERMICULATUM FOR PREPARATION AND CHARACTERIZATION OF MICROCRYSTALLINE CELLULOSE PRODUCED FROM α-CELLULOSE OF KAPOK PERICARPIUM (CEIBA PENTANDRA)

  • HERMAN SURYADI Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia
  • SUTRIYO Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia
  • Mira Junnisa Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia
  • YULIANITA PRATIWI INDAH LESTARI Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia

Abstract

Objective: This study aimed to find psychochemical properties of microcrystalline cellulose (MCC) obtained from α-cellulose kapok pericarpium.
Methods: The cellulase activity was screened by clear zone and sugar reduction method. The enzym from selected mold was purified by diethylaminoethyl (DEAE) chromatography. α-cellulose of kapok pericarpium was hydrolyzed using the purified cellulase enzymes. Microcrystalline cellulose (MCC) identified by Fourier transform infrared (FTIR) spectrometry, and qualitative analysis test. The samples were characterized for pH test, x-ray diffraction (XRD), and particle size analyzer (PSA).
Results: The optimum cellulase activity was shown by Penicillium vermiculatum. It’s clear zone diameter around 3 cm and the cellulase activity was 67.73±0.25 mU/ml. The strongest cellulase activity was detected from 1st fraction (P1) out of 6 column fractions with optimum activity at 1.177±2 mU/ml. The optimal conditions for microcrystalline cellulose (MCC) preparation were at 50 ˚C, for 2 ours, using 20 ml of acetate buffer pH 5 and 2 ml of cellulase enzyme. Microcrystalline cellulose (MCC) obtained at 78% w/w and its FTIR spectrum and x-ray diffractogram similar to reference while the pH of MCC was fulfilled requirements of The United States Pharmacopoeia 2007.
Conclusion: The use of purified enzyme of cellulase has succeded in microcrystalline cellulose (MCC) preparation andmicrocrystalline cellulose (MCC) obtained was 78% w/w, with similar characteristics to reference (Avicel PH 101) and the pH of MCC was fulfilled requirements of The United States Pharmacopoeia 2007.

Keywords: Microcrystalline cellulose, Enzyme purification, Kapok, Enzyme Hydrolyisis, Characterization

Author Biography

Mira Junnisa, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia

Faculty of pharmacy

References

1. Somporn Chaiarakkeji, Apiporn Apirakchaiskul, Kuntinee Suvarnakich, Suda Kiatkamjornwong. Kapok I: Characteristics of kapok fiber as a potential pulp source for papermaking. BioResources 2011;7:475.
2. Maljoltra Bhanu, Keshwani Anu, Kharkwal Harsha. Natural polymer based cling films for food packaging. Int J Pharm Pharm Sci 2015;7: 10.
3. Mardiyati, Rizkiansyah, RR, Steven, Basuki A, Suratman R. Serat kapuk sebagai bahan baku pembuatan mikrokristalin selulosa. Jurnal Sains Materi Indonesia 2016;17:173.
4. Hindi SSZ. Microcrystalline Cellulose: The inexhaustible treasure for pharmaceutical industry. Nanosci Nanotechnol Res 2017;1 Supp 4:17.
5. Naser MY Hasan, Dhaifallah M Almalki, Mohammed JK Althuwaybi, Hassan M Alshehri. Smedds tablet: Compatibility of solid smedds using various pharmaceutical tablet excipients. Int J Pharm Pharm Sci 2016;8:246.
6. SV Shelar, VS Shirolkar, NR Kale. Formulation optimization of promethazine theoclate immediate releasepellets by using extrusion-spheronization technique. Int J App Pharm 2018;10:30.
7. Rasha KD, Myasar Al-Kotaji. Formulation of orally disintegrating tablets of cinnarizine by using direct compression method. Int J App Pharm 2019;11:117.
8. Suryadi H, Sutriyo, Sari HR, Rosikhoh Dianah. Preparation of microcrystalline cellulose from water hyacinth powder by enzymatic hydrolisis using cellulase of local isolate. J Young Pharmacists 2017;9:S20.
9. Megha SV et al. Isolation and purification of cellulase. Int J Sci Nat 2015;6:475.
10. Ire FS, Okoli AO, Ezebuiro V. Production and optimization of cellulase from Penicillium sp. Using corn-cob and pawpaw fibre as subtrates. J Adv Micro 2018;8:4-5.
11. United States Pharmacopoeial Convention. The United States Pharmacopoeia (USP) 30-The National Formulary (NF) 30. Rockville: The United States Pharmacopeial Convention, Inc; 2007.
12. Harmita. Analisis Fisikokimia. Depok: Departemen Farmasi FMIPA Universitas Indonesia; 2006.
13. British Pharmacopoeia Comission. British Pharmacopoeia. Vol 1. London: The Stationary Office; 2002.
14. Suryadi H, Sutriyo,Angeline M, Murti MW. Characterization of microcrystalline cellulose obtained from enzymatic hydrolisis of alpha-cellulose and its application. J Young Pharmacists 2018;10:S88.
15. Kips R et al. Characterization of uranium particles produced by hydrolysis of UF6 using SEM and SIMS. Microsc Microanal 2007;13:158.
16. Rojas J, Lopez A, Guisao S, Ortiz C. Evaluation of several microcrystalline celluloses obtained from agricultural by products. J Adv Pharm Technol Res 2011;2:144-50.
17. Zhang Y-HP, Himmei ME, Mielenz JR. Outlook for cellulose improvement: screening and selection strategies. Biotechnol Adv 2006;24:452.
18. Bonner PLR. Protein Purification. 2nd Ed. New York: Taylor & Francis;2018.
19. Nooralabettu KP. Effective anion exchange chromatoghraphic purification of hepatopancreatic alkaline phosphatase of Red shrimp, Solenocera choprai. Int J Anal Bio-Sci 2014;2:41-50.
20. Deshpande R, Sundvall L, Grundberg H, Germgard U. The influence of different types of bisulfite cooking liquors on pine wood components. Bioresources 2016;11:5961-5962.
21. Nemec M, Wecker L, Hajdas I, Gaggeler H. Alternative methode for cellulose preparation for AMS measurement. Radiocarbon 2010;52:1359.
22. Idrees M, Adnan A, Qureshi A. Optimization of sulfide/sulfite pretreatment of lignocellulosic biomass for lactic acid production. Biomed Res Int 2013:1-9.
23. Charles E, Carraher Jr. Carraher’s Polymer Chemistry. 9th ed. Boca Raton: CRC Press 2016;294.
24. Gibbon John. Technologies for reducing dioxin in the manufacture of bleached wood pulp.United States: DIANE Publishing;1989.
Statistics
94 Views | 102 Downloads
Citatons
How to Cite
SURYADI, H., SUTRIYO, Junnisa, M., & LESTARI, Y. P. I. (2019). POTENTIAL OF CELLULASE OF PENICILLIUM VERMICULATUM FOR PREPARATION AND CHARACTERIZATION OF MICROCRYSTALLINE CELLULOSE PRODUCED FROM α-CELLULOSE OF KAPOK PERICARPIUM (CEIBA PENTANDRA). International Journal of Applied Pharmaceutics, 11(4), 92-97. https://doi.org/10.22159/ijap.2019v11i4.31094
Section
Original Article(s)