STABILITY OF MICROSPHERE OF SARGASSUM PLAGYOPHYLLUM (MERTENS) J. G. AGARDH EXTRACT PRODUCED BY SPRAY DRYING USING MALTODEXTRIN
Objective: This study aimed to determine the stability of microspheres of Sargassum plagyophyllum (brown seaweed) after preparation using spray
drying with maltodextrin DE 10â€“15 and during drying and storage.
Methods: Aqueous extracts of brown seaweed were formulated into microspheres using maltodextrin DE 10â€“15 as a coating agent. For increasing the
stability of polyphenol compounds, spray drying was performed with an inlet temperature of 110Â°C. Four microsphere formulations were produced
using maltodextrin DE 10â€“15 at concentrations of 0%, 5%, 10%, and 15%. The resulting microspheres were then characterized in the assessments of
moisture contents, particle size distributions, pH, total phlorotannin contents, and antioxidant activity, and surface morphology was analyzed using
scanning electron microscope analyses.
Results: Powders that were produced with 0% and 15% maltodextrin were more stable at 4Â°CÂ±2Â°C than at 28Â°CÂ±2Â°C and 40Â°CÂ±2Â°C. At the lowest
temperature, phlorotannin contents were maintained in powders that were prepared with 15% maltodextrin but were decreased by 10% in powders
that were prepared without maltodextrin.
Conclusion: Maltodextrin DE 10â€“15 is a suitable coating agent for dry formulations of S. plagyophyllum powder and maintained stability during spray
drying at 110Â°C and during storage for 2 months at 4Â°CÂ±2Â°C.
polycystum on tyrosinase activity and melanin formation in B16F10
murine melanoma cells. J Ethnopharmacol 2011;137:1183-8.
2. Shin T, Ahn M, Hyun JW, Kim SH, Moon C. Antioxidant marine algae
phlorotannins and radioprotection: A review of experimental evidence.
Acta Histochem 2014;116:669-74.
3. Sathya R, Kanaga N, Sankar P, Jeeva S. Antioxidant properties of
phlorotannins from brown seaweed Cystoseira trinodis (Forsskal) C.
Agardh. Arab J Chem 2011;10:S2608-14.
4. Li Y, Wijesekara I, Li Y, Kim S. Phlorotannins as bioactive agents from
brown algae. Process Biochem 2011;46:2219-24.
5. Kapuge K, Sanjeewa A, Kim E, Son K, Jeon Y. Bioactive properties
and potentials cosmeceutical applications of phlorotannins isolated
from brown seaweeds : A review. J Photochem Photobiol B Biol
6. Fang Z, Bhandari B. Effect of spray drying and storage on the stability
of bayberry polyphenols. Food Chem 2011;129:1139-47.
7. Caliskan G, Dirim SN. The effect of different drying processes and the
amounts of maltodextrin addition on the powder properties of sumac
extract powders. Powder Technol 2016;287:308-14.
8. Zokti JA, Baharin BS, Mohammed AS, Abas F. Molecules green tea
leaves extract: Microencapsulation, physicochemical and storage
stability study. Molecules 2016;21:1-24.
9. Masduqi AF, Izzati M, Prihastanti E. Effect of drying method on
chemical content in sargassum polycystum seaweed [Efek metode
pengeringan terhadap kandungan bahan kimia dalam rumput laut
sargassum polycystum]. Bull Anat dan Fisiol 2014;22:1-9.
10. Ye H, Zhou C, Sun Y, Zhang X, Liu J, Hu Q, et al. Antioxidant activities
in vitro of ethanol extract from brown seaweed Sargassum pallidum.
Eur Food Res Technol 2009;230:101-9.
11. Koivikko R. Brown Algal Phlorotannins Improving and Applying
Chemical Methods. Turku: Department of Chemistry University of
12. Quiros AR, Frecha-Ferreiro S, Vidal-Perez AM, Lopez-
Hernandez J. Antioxidant compounds in edible brown seaweeds. Eur
Food Res Technol 2010;231:495-8.
13. Kim J, Yoon M, Yang H, Jo J, Han D, Jeon YJ, et al. Enrichment and
purification of marine polyphenol phlorotannins using macroporous
adsorption resins. Food Chem 2014;162:135-42.
14. Munâ€™im A, Negishi O, Ozawa T. Antioxidative compounds from
Crotalaria sessiliflora. Biosci Biotechnol Biochem 2003;67:410-4.
15. Molyneux P. The use of the stable free radical diphenylpicryl- hydrazyl
(DPPH) for estimating antioxidant activity. Songklanakarin J Sci
16. Pycia K, Juszczak L, GaÅ‚kowska D, Witczak M, Jaworska G.
Maltodextrins from chemically modified starches as agents affecting
stability and rheological properties of albumin foam. Selected
physicochemical properties. Carbohydr Polym 2016;146:301-9.
17. Caliskan G, Dirim SN. The effects of the different drying conditions
and the amounts of maltodextrin addition during spray drying of sumac
extract. Food Bioprod Process 2013;91:539-48.
18. Tan SP, Kha TC, Parks SE, Stathopoulos CE, Roach PD. Effects of
the spray-drying temperatures on the physiochemical properties of an
encapsulated bitter melon aqueous extract powder. Powder Technol
19. RamÃrez MJ, Giraldo GI, Orrego CE. Modeling and stability of
polyphenol in spray-dried and freeze-dried fruit encapsulates. PowderTechnol 2015;277:89-96.
20. Mishra P, Mishra S, Mahanta CL. Effect of maltodextrin concentration
and inlet temperature during spray drying on physicochemical and
antioxidant properties of amla (Emblica officinalis) juice powder. Food
Bioprod Process 2014;92:252-8.
21. Vijayalakshmi K, Srimathi PK. Investigation of antioxidant potential
of quercetion and hesperidin: An in vitro approach. Asian J Pharm Clin
22. Samran S, Dalimunthe D. The formulation of dry curcuma
(Curcuma xanthorrhiza roxb.) extract microcapsules by spray wet
microencapsulation techniques. Asian J Pharm Clin Res 2018;11:226-9.
23. Amatrejo S, Tanjung HR. Tinospora extract (Tinospora crispa (L.)
Miers.) encapsulation with sodium alginate isolated from brown algae
(Sargassum ilicifolium). Asian J Pharm Clin Res 2018;10:267-9.