ANALYSIS OF LYSATE PLATELET-RICH FIBRIN EFFECTS ON HUMAN DENTAL PULP STEM CELL DIFFERENTIATION THROUGH DENTIN SIALOPHOSPHOPROTEIN EXPRESSION

  • SILVIANA SWASTININGTYAS Conservative Dentistry Residency Program, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
  • ANGGRAINI MARGONO Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
  • DINI ASRIANTI Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
  • RUNY OKTAYANI Conservative Dentistry Residency Program, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
  • INDAH YULIANTO Department of Dermatology, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Central Java, Indonesia.

Abstract

Objective: In vitro, the culture media in which human dental pulp stem cells (hDPSCs) are grown are supplemented with specific growth factors that
induce cell cycle entry and differentiation. Lysate platelet-rich fibrin (L-PRF) is a unique and stable growth factor supplement produced from platelets
lysed by freezing-thawing. In this study, we aimed to analyze the potential effects of L-PRF on hDPSC differentiation.
Methods: We divided hDPSCs isolated from human third molars at the second passage into five culture media groups treated with 1%, 5%, 10%,
and 25% L-PRF or 10% fetal bovine serum (control). After 7 days, we evaluated hDPSC differentiation using an enzyme-linked immunosorbent assay
specific for dentin sialophosphoprotein and Alizarin-Red staining.
Results: None of our analyses revealed any significant differences between the L-PRF- and control-treated cells.
Conclusion: L-PRF could potentially induce the differentiation of hDPSCs in vitro.

Keywords: Lysate platelet-rich fibrin, Differentiation,, Human dental pulp stem cells, Dentin sialophosphoprotein

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SWASTININGTYAS, S., MARGONO, A., ASRIANTI, D., OKTAYANI, R., & YULIANTO, I. (2020). ANALYSIS OF LYSATE PLATELET-RICH FIBRIN EFFECTS ON HUMAN DENTAL PULP STEM CELL DIFFERENTIATION THROUGH DENTIN SIALOPHOSPHOPROTEIN EXPRESSION. International Journal of Applied Pharmaceutics, 12(2), 34-37. https://doi.org/10.22159/ijap.2020.v12s2.OP-43
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