ANTI-CHOLOSETEROL ACTIVITIES OF WHITE (RAPHANUS RAPHANISTRUM) AND RED (RAPHANUS SATIVUS) RADISH ROOTS
Objective: The purpose of this research is to explore the in vitro activities of white (Raphanus raphanistrum) and red (Raphanus sativus) radish root ethanol extract in decreasing cholesterol levels.
Methods: Ultrasonification method was used in obtaining 96% ethanol extract of white and red radish root. The cholesterol levels were analyzed by visible spectrophotometry, which was validated using Lieberman-Buchard reagents. The decreased cholesterol levels were converted into IC50 values.
Results: The results showed that the IC50 of 96% white and red radish root ethanol extracts were 743.7 and 634.7 µg/ml, respectively. The results of statistical analysis using the T-test obtained a significant value greater than the probability value (P) of 0.05.
Conclusion: Therefore, it was concluded that the activities of 96% ethanol extract of white and red radish roots did not have a significant ability to reduce the in vitro cholesterol levels.
2. Moghimipour E, Kooshapour H, Rezaee S, Khalili S, Handali S. In vitro cholesterol-binding affinity of total saponin extracted from Glycyrrhiza glabra. Asian J Pharm Clin Res 2013;7:170-3.
3. Metwally MA, El-Gellal AM, El-Sawaisi SM. Effects of silymarin on lipid metabolism in rats. World Appl Sci 2009;6:1634-7.
4. Okazaki M, Morio Y, Iwai S, Miyamoto K, Sakamoto H, Imai K, et al. Age-related changes in blood coagulation and fibrinolysis in mice fed on a high-cholesterol diet. Exp Anim 1998;47:237–46.
5. Haque M, Islam J, Rahaman A, Selina FA, Azizur M, Rahman MH, et al. Raphanus sativus ameliorates atherogeneic lipid profiles in hypercholesterolemic rats and hypercholesterolemia-associated peroxidative liver damage. J Adv Chem 2014;7:1385-94.
6. Magied MMA, Alian AM, haerrdy L, Hussein MT. The protective effect of white and red radish as hypoglycemic and hypocholesterolemic agents. J Pharm Biol Sci 2016;11:51–61.
7. Shin T, Ahn M, Kim GO, Park SU. Biological activity of various radish species. Orient Pharm Exp Med 2015;15:105–11.
8. Gashaw A, Getachew T. Cholesterol content and free fatty acids in edible oils and health effects: a review. Int J Res Appl Sci Eng Technol 2014;2:120–5.
9. Djamil R, Zaidan S, Butar VB, Pratami DK. Formulasi nanoemulsi ekstrak etanol buah okra (Abelmoschus esculentus (L.) Moench.) dan uji aktifitas antikolesterol secara in vitro. J Ilmu Kefarmasian Indones 2020;18:75–80.
10. Djamil R, Zaidan S, Rahmat D, Pratami DK, Hakim F. Nanoemulsion of okra fruit extract as antidiabetic treatment. Int J Appl Pharm 2020;12:138-42.
11. Farnsworth NR. Biological and phytochemical screening of plants. J Pharm Sci 1966;55:225–76.
12. Brittain HG. editor. Profiles of drug substances, excipients, and related methodology. London: Academic Press; 2020.
13. Yantih N, Methananda A, Harahap Y, Sumaryono W, Rahayu L. Validation of high-performance liquid chromatography for determination of bromelain in pineapple (Ananas comosus (L) Merr) water. Pharmacogn J 2019;11:901-6.
14. Martono Y, Rohman A. Quantitative analysis of stevioside and rebaudioside a in Stevia rebaudiana leaves using infrared spectroscopy and multivariate calibration. Int J Appl Pharm 2019;11:38-42.
15. Lotfy HM. Determination of simvastatin and ezatimibe in combined tablet dosage forms by constant center spectrophotometric method. Int J Pharm Pharm Sci 2012;4:673-9.
16. Adanlawo IG. Tissue lipid profile of rats administered saponin extract from the root of bitter kola. Biochemistry 2013;1:1–4.
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