PHYTOCHEMICAL SCREENING AND ANTI-INFLAMMATORY ACTIVITY OF FRACTIONS FROM SAMBUNG RAMBAT (MICANIA CORDATA) LEAF

  • Zola Efa Harnis Department of Biological Pharmacy, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia.
  • Panal Sitorus Department of Biological Pharmacy, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia.
  • Rosidah Rosidah Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia.

Abstract

Objective: The present study is to evaluate the anti-inflammatory activity of various fractions from Sambung Rambat leaf (Micania cordata) using ethyl acetate, hexane, and water solvent as the solvent.

Methods: Investigation of phytochemical compound was using standard phytochemical screening method, while the anti-inflammatory was using the carrageenan-induced paw edema method in animal (rats) model.

Results: The phytochemical screening showed that the fractions of M. cordata had a lot of phytochemical compounds such as flavonoids, glycosides, steroids, triterpenoids, tannins, and saponins. Anti-inflammatory assessment showed that the strongest activity of anti-inflammatory produced by ethyl acetate fraction with 200 mg/kg BW dose.

Conclusion: This investigation could be concluded that ethyl acetate fraction of M. cordata might be a potential for the treatment of inflammatory.

Keywords: Anti-inflammatory, Carrageenan, Micania cordata, Phytochemical, Screening.

Author Biographies

Zola Efa Harnis, Department of Biological Pharmacy, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia.
Department of Biological Pharmacy
Panal Sitorus, Department of Biological Pharmacy, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia.
Department of Biological Pharmacy
Rosidah Rosidah, Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia.
Department of Pharmacology

References

1. Leelaprakash G, Dass SM. In vitro anti-inflmmatory activity of methanol extract of Enicostemma axillare. Int J Drug Dev Res 2011;3:189-96.
2. Sadique J, Chandra T, Thenmozhi V, Elango V. The anti-inflammatory activity of Enicostemma littorale and Mullogo cerviana. Biochem Med Metab Biol 1987;37:167-76.
3. Paul RK, Jabbar A, Rashid MA. Antiulcer activity of Mikania cordata. Fitoterapia 2000;71:701-3.
4. Ahmed M, Rahman MT, Alimuzzaman M, Shilpi JA. Analgesic sesquiterpene dilactone from Mikania cordata. Fitoterapia 2001;72:979 21.
5. Bhattacharya S, Nag AK. Chemical constituents of the essential oil of Mikania cordata (Burm) B.L. Robinson root extract in rodent. J Essent Oil Res 1992;6:255-9.
6. Bishayee A, Chatterjee M. Anticarciogenic biological response of Mikania cordata: Reflections in hepatic biotransformation systems. Cancer Lett 1994;81:193-200.
7. Chowdhury NS, Alam MB, Zahan R, Sultana S, Nahar K, Haque ME. Antimicrobial and toxicity studies of different fractions of the aerial parts of the Mikania cordata. Int J Pharm Life Sci 2011;2:592-8.
8. Heyne K. Usefull Indonesia Plant. Jakarta: Yayasan Sarana Wana Jaya; 1987. p. 551-2.
9. Wiart C. Ethnopharmacology of Medicinal Plants: Asia and the Pacific. Totowa: Humana Press Inc.; 2006. p. 36.
10. Ditjen PO. Farmakope Indonesia. Vol. 4. Jakarta: Depkes RI; 1995. p. 896-8.
11. Basset J, Denney RC, Jeffrey GH, Mendham J. Buku Ajar Vogel: Kimia Analisis Kuantitatif Anorganik. 4th ed. Jakarta: EGC; 2018.
12. Sinaga SM, Iksen I, Haro G, Wardany S. Potency of chives (Allium schoenoprasum L.) leaves infuse as inhibitor calcium lithogenesis on urinary tract. Asian J Pharm Clin Res 2018;11:77-80.
13. Indonesia’s Department of Health. Materia Medika. Vol. 6. Jakarta: National Agency of Drug and Food Control; 1995. p. 297-307.
14. Farnsworth NR. Biologycal and phytochemical screening of plants. J Pharm Sci 1996;55:225-76.
15. Harbone JB. Phtyochemistry method. ITB 1987;2:6, 49.
16. Haro G, Sinaga SM, Iksen I, Nerdy N, Theerachetmongkol S. Protective effects of chives leaves (Allium schoenoprasum L.) infusion against ethylene glycol and ammonium chloride induced nephrolithiasis in rats. J Appl Pharm Sci 2017;7:222-5.
17. Winter CA, Risley EA, Nuss GW. Carrageenin induced oedema in hindpaws of the rats as an assay of anti-inflammatory drugs. Proc Soc Exp Biol Med 1962;111:544-7.
18. Yu D, Yuan Y, Jiang L, Tai Y, Yang X, Hu F, et al. Anti-inflammatory effects of essential oil in Echinacea purpurea L. Pak J Pharm Sci 2013;26:403-8.
19. Vinueza D, Lopez E, Acosta K, Abdo S. Assessment of anti-inflammaotry activity and cytotoxicity of freeze dried hydroalcoholic extract of Bidens andicola on isolated neutrophils. Asian J Pharm Clin Res 2017;10:160-3.
20. Ullah AH, Akter L, Zaman S, Juhara F, Tareq SM, Bhattacharjee R, et al. Anti-inflmammatory and analgesic activities of methanolic seed extract of Sterculia villosa Roxb. Asian J Pharm Clin Res 2015;8:247 51.
21. Laaboudi W, Ghanam J, Aissam H, Merzouki M, Benlemlih M. Anti-inflammatory and analgesic activities of olive tree extract. Int J Pharm Pharm Sci 2016;8:414-9.
22. Shaikh S, Joshi YM, Kadam V. Comparative study of anti-inflammatory activity if aqueous and methanolic extracts of Hibiscus cannabinus leaf (Malvaceae). Int J Pharm Pharm Sci 2016;8:64-8.
Statistics
123 Views | 226 Downloads
How to Cite
Harnis, Z. E., P. Sitorus, and R. Rosidah. “PHYTOCHEMICAL SCREENING AND ANTI-INFLAMMATORY ACTIVITY OF FRACTIONS FROM SAMBUNG RAMBAT (MICANIA CORDATA) LEAF”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 11, no. 8, Aug. 2018, pp. 199-01, doi:10.22159/ajpcr.2018.v11i8.26083.
Section
Original Article(s)