EFFECT OF HARVESTING TIME AT MORNING, AFTERNOON, AND EVENING ON NITRATE AND NITRITE LEVEL IN SPINACH (AMARANTHUS TRICOLOR L.) AND MUSTARD (BRASSICA RAPA L.)
Keywords:Harvesting time, Nitrate, Nitrite, Spinach, Mustard
Â Objective: The objective of this study is to determine the effect of harvesting time at morning, afternoon, and evening on nitrate and nitrite level in spinach and mustard.
Methods: Nitrite identification was done using sulfanilic acid reagent and N-(1-naphthyl) ethylenediamine dihydrochloride. Identification of nitrate was done using reagent ferrous sulfate. Determination of nitrites level was performed visible spectrophotometry using N-(1-naphthyl) ethylenediamine dihydrochloride at maximum wavelength of 540 nm. Nitrate determination is taken with the same method but started with reduction process from nitrate into nitrite using Zn powder in acid condition and then measured as nitrite.
Results: Research result shows that there is a change of nitrate and nitrite level from the spinach harvested at morning, afternoon, and evening.
Conclusion: Spinach and mustard are better harvested in the morning because it contains nitrite less than in spinach picked afternoon and evening. Level of nitrite increases from morning to afternoon and decreases from afternoon to evening. However, the level of nitrate decreases from morning to afternoon and increases from afternoon to evening.
Dias JS. Nutritional quality and health benefits of vegetables. Food Nutr Sci 2012;3:1354-74.
Saparinto C. Grow Your Own Vegetables. Yogyakarta: Lily Publisher; 2013.
Akbar R. Various Pharmacies Plant Live Around us. Jakarta: One Books; 2015.
Silalahi J. Problem of nitrite and nitrate in food. Med J Ked Ind 2005;31:460-1.
Amri B. Effect of boiling and storage time on nitrate and nitrite levels on spinach (Amaranthus tricolor L.). Medan: Fakultas Farmasi; 2016.
Asmaradhani D. Effect of fertilization on nitrate and nitrite level on kale (Ipomoea reptana poir). Medan: Fakultas Farmasi; 2016.
Keeton JT. History of Nitrate an Nitrite in food. New York: Humana Press; 2011.
Murthy R, Dougherty PM, Zarnoch SJ, Allen HL. Effects of carbon dioxide, fertilization, and irrigation on photosynthetic capacity of loblolly pine trees. Tree Physiol 1996;16:537-46.
Latifa IC, Anggarwulan E. Nitrogen content, nitrate reductase activity, and biomass of kimpul (Xanthosoma sagittifolium) on shade and nitrogen fertilizer variation. Nusantara Biosci 2009;1:65-71.
Sudjana N. Statistic Method. 6th ed. Bandung: Tarsito; 2005.
Harmita. Method Validation Implementation Guidelines and Calculations. Jakarta: Department of Pharmacy, Faculty of Mathematics and Natural Sciences, University of Indonesia; 2004.
Hess J. Association Official Methods of Analytical Chemists. 17th ed. Virginia: AOAC Inc.; 2000.
Nerdy, Putra ED, Tjahjono DH. Development and validationof high performance liquid chromatography mass spectrometry method for determination of rifampicin, isoniazidand pyrazinamide from tablet preparation. Int J Pharmtech Res 2014;6:1647-64.
Hill MJ. Nitrates and Nitrites From Food and Water in Relation to Human Disease. Cambridge: Woodhead Publishing Limited; 1996.
Maynard DN, Barker AV, Minotti PL, Peck NH. Nitrate Accumulation in Vegetables. J Adv Agron 1976;28:71-118.
Nerdy. Development and validation of ultraviolet spectrophotometric method for determination of mebhydrolin napadisylate in tablet preparations. Asian J Pharm Clin Res 2017;10:367-72.
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