NANOPARTICLES OF BROWN SEAWEED (SARGASSUM POLYCYSTUM) EXTRACT AND ITS ANTIOXIDANT ACTIVITY IN RATS FED HIGH-FAT DIET

Authors

  • SARAH ZAIDAN Faculty of Pharmacy, Universitas Pancasila, South Jakarta, 12640, Indonesia
  • SYAMSUDIN ABDILLAH Postgraduate Program, Faculty of Pharmacy, Universitas Pancasila, South Jakarta
  • DIAH KARTIKA PRATAMI National Metabolomics Collaborative Research Center, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, West Java, Indonesia https://orcid.org/0000-0003-1052-5946
  • SYIFA AULIA UTAMI Faculty of Pharmacy, Universitas Pancasila, South Jakarta, 12640, Indonesia

DOI:

https://doi.org/10.22159/ijap.2022v14i4.44677

Keywords:

Brown Seaweed, Nanoparticles, Antioxidants activity, Sargassum polycystum

Abstract

Objective: This research aimed to formulate the nanoparticles of brown seaweed (Sargassum polycystum) and determine its antioxidant activity in rats on high-fat diets (HFD) with the parameters of superoxide dismutase (SOD), catalase, and malondialdehyde (MDA).

Methods: The nanoparticles of brown seaweed extract (NBSE) were formulated with chitosan and tripolyphosphate polymers using the ionic gelation method. The NBSE was evaluated for its particle size and potential zeta. Twenty-four male Wistar rats were divided into six groups of four rats each. One group of normal control groups was given a standard diet as a baseline. Rats were induced with a high-fat diet for 35 d. The HFD rats were divided into five equal subgroups of four animals each: an untreated group as a negative control; a group treated with vitamin E as a positive control, and three NBSE treatment groups that had oral administration of 50, 100, or 200 mg/kg BW, respectively, for 14 d. On days 0, 14, 35, and 49, blood samples were taken to determine antioxidant activity against antioxidant parameters such as MDA, SOD, and catalase.

Results: The NBSE has a particle size of 891.2±44.3 d. nm, a polydispersity index of 0.3820.03, and a zeta potential value of 11.7±0.2 mV with a positive charge. The oral administration of NBSE prevented oxidative stress on HFD rats by increasing SOD and catalase, with the highest inhibition percentage SOD parameters were 196.71% and catalase parameters were 218.73%. Formation of MDA was prevented by all doses with the highest inhibition percentage MDA parameters were 57.90%.

Conclusion: The NBSE has antioxidant activity and could prevent oxidative stress in HFD rats.

Downloads

Download data is not yet available.

References

Trinder M, Li X, DeCastro ML, Cermakova L, Sadananda S, Jackson LM, et al. Risk of premature atherosclerotic disease in patients with monogenic versus polygenic familial hypercholesterolemia. J Am Coll Cardiol. 2019;74(4):512–22.

Rizk MZ, El-sherbiny M, Borai IH, Ezz MK, Aly HF, Matloub AA, et al. Sulphated polysaccharides (SPS) from the green alga Ulva fasciata extract modulates liver and kidney function in high fat diet-induced hypercholesterolemimic rats. Int J Pharm Pharm Sci. 2016 Jun 1;8(6 SE-Original Article(s)):43–55.

Paknahad Z, Moosavian SP, Mahdavi R, Rajabi P. The effects of olive oil and cholesterol enriched diets on aortic fatty streak development and lipid peroxidation in rabbits. Nutr Health. 2021;02601060211022260.

Teimouri M, Yeganeh S, Mianji GR, Najafi M, Mahjoub S. The effect of Spirulina platensis meal on antioxidant gene expression, total antioxidant capacity, and lipid peroxidation of rainbow trout (Oncorhynchus mykiss). Fish Physiol Biochem. 2019;45(3):977–86.

Sharma H, Joshi A, Lad H, Bhatnagar D. Anti-Oxidative, anti-inflammatory and anti-atherosclerotic effect of taurine on hypercholesterolemia induced atherosclerotic rats. Int J Pharm Pharm Sci. 2018 Mar 1;10(3 SE-Original Article(s)):145–50.

Badarinath A V., Mallikarjuna Rao K, Madhu Sudhana Chetty C, Ramkanth S, Rajan TVS, Gnanaprakash K. A review on In-vitro antioxidant methods: Comparisions, correlations and considerations. Int J PharmTech Res. 2010;2(2):1276–85.

Asem N, Abdul Gapar NA, Abd Hapit NH, Omar EA. Correlation between total phenolic and flavonoid contents with antioxidant activity of Malaysian stingless bee propolis extract. J Apic Res. 2020;59(4):437–42.

Djamil R, Zaidan S, Rahmat D, Pratami DK, Hakim F. Nanoemulsion of okra fruit extract as antidiabetic treatment. Int J Appl Pharm. 2020;12(5):138–42.

Djamil R, Rahmat D, Zaidan S, Latifah MN. Anticholesterol activity of okra fruit extract (Abelmoschus esculentus ( L ) Moench ) and its nanoemulsion in vivo. Pharmacogn J. 2020;12(2):1–5.

Silvana Naiu A, Berhimpon S, Iwan Montolalu R, Kawung NJ, Suptijah P. The effect of hcl-thermal pressure hydrolysis and high-speed destruction of chitin on particle size distribution and functional group of nano-chitin compound. Curr Res Nutr Food Sci. 2020;8(1):197–205.

Makhlouf A-MA, Mahmoud AM, Ibrahim RG, Aziz YSA. Effects of Vitamin D and simvastatin on inflammatory and oxidative stress markers of high-fat diet-induced obese rats. J Sci Res Med Biol Sci. 2021;2(3):39–50.

Marília d’ AF, Souto OP, Pereira DFS, Jacobsen FT, P. PCM, Quintana OS, et al. Eugenol derivatives as potential anti-oxidants: is phenolic hydroxyl necessary to obtain an effect? J Pharm Pharmacol. 2013 Dec 27;66(5):733–46.

Tsikas D. Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples: Analytical and biological challenges. Anal Biochem. 2017;524:13–30.

Ajiboye TO. Standardized extract of Vitex doniana Sweet stalls protein oxidation, lipid peroxidation and DNA fragmention in acetaminophen-induced hepatotoxicity. J Ethnopharmacol. 2015;164:273–82.

Xia H, Wang J, Xie X, Xu L, Tang S. Green tea polyphenols attenuate hepatic steatosis, and reduce insulin resistance and inflammation in high-fat diet-induced rats. Int J Mol Med. 2019;44(4):1523–30.

Mubasheera MG DHPM. Medicinal Use of an A Brown Seaweed Ancient Algae Sargassum polycystum : A Review. 2021;01(02):29–37.

Huguet-Casquero A, Gainza E, Pedraz JL. Towards Green Nanoscience: From extraction to nanoformulation. Biotechnol Adv. 2021;46(November 2020):107657.

Pratiwi G, Martien R, Murwanti R. Chitosan nanoparticle as a delivery system for polyphenols from meniran extract (Phyllanthus niruri l.): Formulation, optimization, and immunomodulatory activity. Int J Appl Pharm. 2019;11(2):50–8.

Mendez-Pfeiffer P, Juarez J, Hernandez J, Taboada P, Virués C, Valencia D, et al. Nanocarriers as drug delivery systems for propolis: A therapeutic approach. J Drug Deliv Sci Technol. 2021;65(March).

Baek EJ, Garcia C V., Shin GH, Kim JT. Improvement of thermal and UV-light stability of β-carotene-loaded nanoemulsions by water-soluble chitosan coating. Int J Biol Macromol. 2020;165:1156–63.

Arozal W, Louisa M, Rahmat D, Chendrana P, Sandhiutami NMD. Development, characterization and pharmacokinetic profile of chitosan-sodium tripolyphosphate nanoparticles based drug delivery systems for curcumin. Adv Pharm Bull. 2021;11(1):77.

Jurić T, Mićić N, Potkonjak A, Milanov D, Dodić J, Trivunović Z, et al. The evaluation of phenolic content, in vitro antioxidant and antibacterial activity of Mentha piperita extracts obtained by natural deep eutectic solvents. Food Chem. 2021;362.

Pratami DK, Mun A, Sundowo A, Sahlan M, Pratami DK, Mun A. Phytochemical Profile and Antioxidant Activity of Propolis Ethanolic Extract from Tetragonula Bee. Pharmacogn J. 2018;10(1):73–80.

Ellamie AM, Fouda WA, Ibrahim WM, Ramadan G. Dietary supplementation of brown seaweed (Sargassum latifolium) alleviates the environmental heat stress-induced toxicity in male Barki sheep (Ovis aries). J Therm Biol. 2020;89:102561.

Alvarez-Suarez JM, Giampieri F, Battino M. Honey as a source of dietary antioxidants: structures, bioavailability and evidence of protective effects against human chronic diseases. Curr Med Chem. 2013;20(5):621–38.

Johnson M, Kanimozhi SA, Malar TRJJ, Shibila T, Freitas PR, Tintino SR, et al. The antioxidative effects of bioactive products from Sargassum polycystum C. Agardh and Sargassum duplicatum J. Agardh against inflammation and other pathological issues. Complement Ther Med. 2019;46:19–23.

Belhadj Slimen I, Najar T, Ghram A, Abdrrabba M. Heat stress effects on livestock: molecular, cellular and metabolic aspects, a review. J Anim Physiol Anim Nutr (Berl). 2016;100(3):401–12.

Ajith TA, Hema U, Aswathy MS. Zingiber officinale Roscoe prevents acetaminophen-induced acute hepatotoxicity by enhancing hepatic antioxidant status. Food Chem Toxicol. 2007;45(11):2267–72.

Krishnan N, Muthukrishnan S. Effect of Nigella sativa seed extract on carbon tetrachloride-induced hepatotoxicity in rats. J Acute Med. 2012;2(4):107–13.

Gharib F, Zeid IM, Salem O, Ahmed EZ. Effects of Sargassum latifolium extract on growth, oil content and enzymatic activities of rosemary plants under salinity stress. Life Sci J. 2014;11(10):933–45.

Kim HJ, Kim A, Herath KHINM, Mihindukulasooriya SP, Jeon Y-J, Kim HJ, et al. Antioxidant potential of Sargassum horneri extracts in the liver of mice with PM-induced asthma. Korean J Food Sci Technol. 2021;53(5):535–43.

Published

28-05-2022

How to Cite

ZAIDAN, S., ABDILLAH, S., PRATAMI, D. K., & UTAMI, S. A. (2022). NANOPARTICLES OF BROWN SEAWEED (SARGASSUM POLYCYSTUM) EXTRACT AND ITS ANTIOXIDANT ACTIVITY IN RATS FED HIGH-FAT DIET. International Journal of Applied Pharmaceutics, 14(4). https://doi.org/10.22159/ijap.2022v14i4.44677

Issue

Section

Original Article(s)

Most read articles by the same author(s)

1 2 > >>