COMPARATIVE ANALYSIS OF SILVER NANOPARTICLES PREPARED BY CHEMICAL AND GREEN SYNTHETIC ROUTE
Keywords:Hydrazine hydrate, Desmodium gangeticum (DG), Silver nanoparticle, Zeta potential, Antimicrobial
Objective: The rapid progress in nanotechnology was always accompanied by their toxic impaction the environment and human health, which creates an urge for researchers to find an alternative approach. In the present work, silver nanoparticles (AgNPs) synthesized through chemical (hydrazine hydrate) and green (aqueous Desmodium gangeticum root extract) route were compared for their biological activity and toxic behavior.
Methods: AgNPs were characterized by different techniques like UV-Visible Spectrophotometry, X-ray Diffractometry (XRD), Fourier transform infra-red spectroscopy (FTIR), Zeta Analyzer, Thermo Gravimetric Analysis (TGA) and Scanning electron microscopy (SEM). The free radical scavenging potential was measured through DPPH, Superoxide scavenging assay and Total phenol content and antimicrobial activity by disc diffusion method. The cytotoxicity was examined by LDH assay.
Results: Characterization showed no significance change in the nature of nanoparticles prepared. AgNPs prepared by biological method showed high free radical scavenging potential, better antimicrobial activity and less-toxic to epithelial cells.
Conclusion: Bioreducing and biostabilizing properties of the plant makes the synthesized nanoparticles to possess the necessary features to be applied in biological applications.
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