SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL EVALUATION OF IRON OXIDE NANOPARTICLES PREPARED BY DESMODIUM GANGETICUM ROOT AQUEOUS EXTRACT

  • Santoshi V SASTRA University
  • Shakila Banu A SASTRA University
  • Gino A Kurian Vascular Biology Laboratory, School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu

Abstract

Objective: Synthesis, characterization and biological evaluation of iron oxide nanoparticles prepared by Desmodium gangeticum root aqueous extract.

Methods: Iron Oxide nanoparticles (FeO NPs) were synthesized by the reduction of iron chlorides using phyto-constituents as bio-reductants. The synthesis of FeO NPs was confirmed by UV-Visible spectrophotometry and the average particle size was determined by X-ray diffractometry (XRD). Morphology of the synthesized FeO NPs was identified using Scanning Electron Microscopy (SEM). Further characterization of FeO NPs was done to study its nature by FTIR and TGA. Biological activity of FeO NPs was analyzed through its free radical scavenging activity and antimicrobial effect. Further, cytotoxicity was examined against LLC-PK1 cell lines using LDH assay.

Results: Thus synthesized particles exhibit rhombohedra crystalline phase. Morphology was identified as spherical and particle size ranges from 25 to 35 nm in diameter with an average size of 30 nm analyzed from SEM micrograph. FeO NPs showed a comparable antimicrobial activity as that of control drug (ciprofloxacin) towards Gram positive bacteria. Iron oxide nanoparticles were also found to be non-toxic by the LDH assay

Conclusion: Green synthesis of Fe nanoparticles using Desmodium gangeticum root aqueous extract was considered to be relatively safe and particles possesses significant biological activity.


 

Keywords: Desmodium gangeticum, FeO Nanoparticles, LLC-PK1 cell line, Antimicrobial agent, Antioxidant, Cytotoxicity

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How to Cite
V, S., S. B. A, and G. A. Kurian. “SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL EVALUATION OF IRON OXIDE NANOPARTICLES PREPARED BY DESMODIUM GANGETICUM ROOT AQUEOUS EXTRACT”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 13, May 2015, pp. 75-80, https://innovareacademics.in/journals/index.php/ijpps/article/view/3997.