GREEN AND CHEMICALLY SYNTHESIZED COPPER OXIDE NANOPARTICLES-A PRELIMINARY RESEARCH TOWARDS ITS TOXIC BEHAVIOUR

  • Lakshmi Praba K Vascular Biology Laboratory, School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu
  • Jayashree M Vascular Biology Laboratory, School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu
  • Shakila Banu K Vascular Biology Laboratory, School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu
  • Gino A Kurian Vascular Biology Laboratory, School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu

Abstract

Objectives: Metal oxide nanoparticles have been widely explored in various applications like biosensors, solar cells, biomedical applications in the recent times. However, biological applications of these nanoparticles needs to be low compatible and this is often found as a limitation. The present study aims to compare CuO nanoparticles prepared by chemical and green route to evaluate its suitability for biological applications.

Methods: CuO nanoparticles prepared by both methods were characterized by using XRD, UV-Visible spectroscopy, FTIR, ZETA for phase structure, size, the functional group presence, thermal stability and surface charge stability respectively. Free radical scavenging potential and phenolic contents, was assessed for biologically prepared CuO nanoparticles.

Results: Green synthesized CuO nanoparticles were found to have higher free radical scavenging potential with good colloidal stability and was also found to have well defined monodisperse nature as compared to chemically synthesized CuO nanoparticles. Evaluation of toxicity in cell line LLC PK1 was found to be slightly toxic.

Conclusion: Green synthesized CuO nanoparticles was found to have good stability when compared to chemically synthesized ones showing that they possess desired attributes to be used for biomedical applications.

 

Keywords: CuO nanoparticles, Chemical synthesis, Desmodium gangeticum, X-ray diffraction, Crystal structure

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Author Biographies

Lakshmi Praba K, Vascular Biology Laboratory, School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu

MSc. Student

School of Chemcial & Biotechnology

SASTRA University

Jayashree M, Vascular Biology Laboratory, School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu

M.Tech Student

School of Chemcial & Biotechnology

SASTRA University

Shakila Banu K, Vascular Biology Laboratory, School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu

PhD Student

School of Chemcial & Biotechnology

SASTRA University

Gino A Kurian, Vascular Biology Laboratory, School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu

Senior Assistant Professor

School of Chemcial & Biotechnology

SASTRA University

References

1. Chattopadhyay DP, Patel BH. Preparation, Characterization and Stabilization of Nanosized Copper particle. Int J Pure Appl Sci Technol 2012;9(1):1-8.
2. Zhiwei Huang, Fang Cui, Haixiao Kang, Jing Chen, Xinzhi Zhang, Chungu Xia. Highly dispersed silica-supported copper nanoparticles prepared by precipitation− gel method: a simple but efficient and stable catalyst for glycerol hydrogenolysis. Chem Mater 2008;20:5090–9.
3. Ponce AA, Klabunde KJ. Chemical and catalytic activity of copper nanoparticles prepared via metal vapor synthesis. J Mol Catal A: Chem 2005;225:1-6.
4. Thi My Dung Dang, Thi Tuyet Thu Le, Eric Fribourg-Blanc, Mau Chien Dang. Synthesis and optical properties of copper nanoparticles prepared by a chemical reduction method. Adv Natl Sci: Nanosci Nanotechnol 2011;2:015009.
5. Sastry MA, Absar IK, Kumar M, Rajiv. Biosynthesis of metal nanoparticles using fungi and actinomycete. Curr Sci 2003;85:162-70.
6. Ramanathan R, Bhargava, Suresh K, Bansal V. Biological synthesis of copper/copper oxide nanoparticles. RSC Adv 2014;4:37816.
7. Iravani S. Green synthesis of metal nanoparticles using plants. Green Chem 2011;3(10):2638-50.
8. Kurian GA, Suryanarayanan, Srilalitha R, Archana R, Padikkala J. Antioxidant effects of ethyl acetate extract of Desmodium gangeticum root on myocardial ischemia reperfusion injury in rat hearts. Chin Med 2011;5:1-7.
9. Rathi A, Rao CH, Ravishankar V, Mehrotra S. Anti-inflammatory and anti-nociceptive activity of the water decoction Desmodium gangeticum. J Ethnopharmacol 2004;9:259-63.
10. Kurian GA, Yagnesh N, Kishan R, Sanchit Paddikkala J. Methanol extract of Desmodium gangeticum roots preserves mitochondrial respiratory enzymes, protecting rat heart against oxidative stress induced by reperfusion injury. J Pharm Pharmacol 2008;60:523-50.
11. Kurian GA, Sachu P, Thomas V. Effect of aqueous extract of the Desmodium gangeticum DC root in the severity of myocardial infarction. J Ethnopharmacol 2005;97:457-61.
12. Lai SC, Peng WH, Huang SC, Ho YL, Huang TH, Lai ZR, et al. Analgesic and anti-inflammatory activities of methanol extract from Desmodium triflorum DC in mice. Am J Chin Med 2009;37:573-88
13. Garcia EJ, Oldoni TL, Alencar SM, Reis A, Loguercio AD, Grande RH. Antioxidant activity by DPPH assay of potential solutions to be applied on bleached teeth. Braz Dent J 2012;23:23-7.
14. Patel A, Patel A, Patel A, Patel NM. Determination of polyphenols and free radical scavenging activity of Tephrosia purpurea linn leaves (Leguminosae). Pharmacogn Res 2010;2:152.
15. Shajiselvin C, Muthu D, Kottai A. In-vitro free radical scavenging activity of various extracts of whole plant of Borreria hispida (Linn). Arch Appl Sci Res 2010;2:54.
16. Prabhu BM, Ali SF, Murdock RC, Hussain SM, Srivatsan M. Copper nanoparticles exert size and concentration dependent toxicity on somato sensory neurons of rat. Nanotoxicol 2010;4:150–60.
17. Swarnkar RK, Singh SC, Gopal R. Effect of aging on copper nanoparticles synthesized by pulsed laser ablation in water: structural and optical characterizations. Bull Mater Sci 2011;34:1363-9.
18. Das R, Nath SS, Bhattacharjee R. Synthesis of linoleic acid capped copper nanoparticles and their fluorescence study. J Fluoresc 2011;21:1165-70.
19. Sengul M, Yildiz H, Gungor N, Cetin B, Eser Z, Ercisli S. Total phenolic content, antioxidant and antimicrobial activities of some medicinal plants. Pakistan J Pharm Sci 2009;22(1):102-6.
20. David O. Saponins in food—A review. Food Chem 1981;1:19-40.
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How to Cite
K, L. P., J. M, S. B. K, and G. A. Kurian. “GREEN AND CHEMICALLY SYNTHESIZED COPPER OXIDE NANOPARTICLES-A PRELIMINARY RESEARCH TOWARDS ITS TOXIC BEHAVIOUR”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 13, May 2015, pp. 156-60, https://innovareacademics.in/journals/index.php/ijpps/article/view/3868.