ONE STEP SYNTHESIS OF IRON OXIDE NANOPARTICLES VIA CHEMICAL AND GREEN ROUTEâ€“AN EFFECTIVE COMPARISON
Objective: Magnetic Iron oxide nanoparticles (IONP) are of potential use in the field of biomedical, bioengineering particularly in vivo applications like tissue repair, drug delivery. However, biocompatibility of the nanoparticles is of great concern. Hence in this manuscript, we compare suitability using IONP prepared by two different routes namely chemical and green, for the biological applications.
Methods: In the green route, Desmodium gangeticum root extract was used as the reducing agent with no specific capping agent for the synthesis of nanoparticles unlike chemical route, where propylene glycol was used. The synthesised nanoparticles were characterized and compared by UV-Vis spectrophotometry, X-ray Diffractometry (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Zeta analyser and Vibrating Sample Magnetometry (VSM).
Results: The results were similar except for that the size of green synthesised IONP was reduced and possesses even distribution (i.e. mono dispersed). Biological activity, as assessed by its free-radical scavenging potential and anti-microbial effect was found to be better in the case of green IONP. Toxicity studies using LLC-PK1 cell line shows relatively low toxicity of green synthesised nanoparticles.
Conclusion: Biologically synthesized IONPshow significant antioxidant effect,retains magnetic behaviour and found to be less toxic, therby proving its compatible nature as required in biomedical applications.
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