• Alagumira Meyyappan SASTRA University
  • Shakila Banu A SASTRA University
  • Gino A Kurian School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu


Objective: Magnetic Iron oxide nanoparticles (IONP) are of potential use in the field of biomedical, bioengineering particularly in vivo applications like tissue repair, drug delivery. However, biocompatibility of the nanoparticles is of great concern. Hence in this manuscript, we compare suitability using IONP prepared by two different routes namely chemical and green, for the biological applications.

Methods: In the green route, Desmodium gangeticum root extract was used as the reducing agent with no specific capping agent for the synthesis of nanoparticles unlike chemical route, where propylene glycol was used. The synthesised nanoparticles were characterized and compared by UV-Vis spectrophotometry, X-ray Diffractometry (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Zeta analyser and Vibrating Sample Magnetometry (VSM).

Results: The results were similar except for that the size of green synthesised IONP was reduced and possesses even distribution (i.e. mono dispersed). Biological activity, as assessed by its free-radical scavenging potential and anti-microbial effect was found to be better in the case of green IONP. Toxicity studies using LLC-PK1 cell line shows relatively low toxicity of green synthesised nanoparticles.

Conclusion: Biologically synthesized IONPshow significant antioxidant effect,retains magnetic behaviour and found to be less toxic, therby proving its compatible nature as required in biomedical applications.


Keywords: Desmodium gangeticum, VSM, Antioxidant, Antimicrobial, Cytotoxicity


Download data is not yet available.


1. Haubold T, Bohn R, Birringer R, Gleiter H. Nanocrystalline intermetallic compounds—structure and mechanical properties. Mater Sci Engg A 1992;153:679-83.
2. Jain TK, Richey J, Strand M, Leslie-Pelecky DL, Flask CA, Labhasetwar V. Magnetic nanoparticles with dual functional properties: drug delivery and magnetic resonance imaging. Biomater 2008;29:4012-21.
3. Mok H, Zhang M. Superparamagnetic iron oxide nanoparticle-based delivery systems for biotherapeutics. Expert Opin Drug Delivery 2013;10:73-87.
4. De Jong WH, Borm PJ. Drug delivery and nanoparticles: applications and hazards. Int J Nanomed 2008;3:133.
5. Barhoumi L, Dewez D. Toxicity of superparamagnetic iron oxide nanoparticles on green alga chlorella vulgaris. Bio Med Res Int 2013;2013:1-11.
6. Kurian GA, Yagnesh N, Kishan RS, Paddikkala J. Methanol extract of Desmodium gangeticum roots preserves mitochondrial respiratory enzymes, protecting rat heart against oxidative stress induced by reperfusion injury. J Pharm Pharmacol 2008;60:523-30.
7. Kurian GA, Paddikkala J. Oral delivery of insulin with Desmodium gangeticum root aqueous extract protects rat hearts against ischemia reperfusion injury in streptozotocin induced diabetic rats. Asian Pac J Trop Med 2010;3:94-100.
8. Srivastava P, Singh VK, Singh BD, Srivastava G, Misra BB, Tripathi V. Screening and identification of salicin compound from Desmodium gangeticum and its in vivo anticancer activity and docking studies with cyclooxygenase (COX) Proteins from Mus musculus. J Proteomics Bioinf 2013;6:109-24.
9. Sun P, Zhang H, Liu C, Fang J, Wang M, Chen J, et al. Preparation and characterization of Fe3O4/CdTe magnetic/fluorescent nanocomposites and their applications in immuno-labeling and fluorescent imaging of cancer cells. Langmuir 2009;26:1278-84.
10. Senthil M, Ramesh C. Biogenic synthesis of Fe3O4 nanoparticles using Tridax procumbens leaf extract and its antibacterial activity on Pseudomonas aeruginosa. Digest J Nanomater Biostruct 2012;7:1655-60.
11. Chen Y, Xie MY, Nie SP, Li C, Wang YX. Purification, composition analysis and antioxidant activity of a polysaccharide from the fruiting bodies of Ganoderma atrum. Food Chem 2008;107:231-41.
12. Singh N, Rajini P. Free radical scavenging activity of an aqueous extract of potato peel. Food Chem 2004;85:611-6.
13. Zhao H, Douglas EP, Harrison BS, Schanze KS. Preparation of CdS nanoparticles in salt-induced block copolymer micelles. Langmuir 2001;17:8428-33.
14. Rahman MM, Jamal A, Khan SB, Faisal M. Characterization and applications of as-grown β-Fe2O3 nanoparticles prepared by hydrothermal method. J Nanopart Res 2011;13:3789-99.
15. Chatterjee J, Haik Y, Chen CJ. Size dependent magnetic properties of iron oxide nanoparticles. J Magn Magn Mater 2003;257:113-8.
16. Misra S, Mathur P, Pant R, Gupta AK. Nanocrystalline magnetic alloy thin films, Indian J Eng Mater S 2004;11:319-22.
17. Uttara B, Singh AV, Zamboni P, Mahajan R. Oxidative stress and neurodegenerative diseases: a review of upstream and downstream antioxidant therapeutic options. Curr Neuropharmacol 2009;7:65.
568 Views | 3327 Downloads
How to Cite
Meyyappan, A., S. B. A, and G. A. Kurian. “ONE STEP SYNTHESIS OF IRON OXIDE NANOPARTICLES VIA CHEMICAL AND GREEN ROUTE–AN EFFECTIVE COMPARISON”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 13, May 2015, pp. 70-74,