ANTIHYPERURICEMIC ACTIVITY OF FOUR PLANTS ANNONACEAE USING HYPERURICEMIC RATS MODEL AND ENZYME ASSAY
Objective: The aim of the research is to study the antihyperuricemic activity of Annona muricata L, Annona squamosa L, Annona reticulata L, and
Stelechocarpus burahol (Bl.) Hook. F. & Th. (Annonaceae).
Methods: The dried powdered leaves were extracted using ethanol by maceration method. The ethanolic extracts of four plants were administered
orally to potassium oxonate-induced rats. Serum samples were collected from rats at the 1
hr after drug administration. Enzyme assay was done
by xanthine oxidase (XO). The XO inhibitory activity was measured by UV-Vis spectrophotometry, and the degree of enzyme inhibition was determined
by measuring the absorbance of uric acid formation.
Results: The result of this study revealed that all of the ethanolic extracts had a significant effect on hyperuricemic rats, and S. burahol extract
decreased uric acid level was equal to level normal control. However, the antihyperuricemic activity was not parallel with XO inhibitor activity which
showed weak activity with IC
of A. reticulata was 171.73 Âµg/ml, while IC
of A. muricata, A. squamosa, and S. burahol more than 200 Âµg/ml.
Conclusion: The ethanolic extract of S. burahol was more potential than three other extracts in decreasing in uric acid levels. The results scientifically
confirmed that S. burahol can be developed as potential antihyperuricemic agents.
Keywords: Antihyperuricemia, Annona muricata, Annona squamosa, Annona reticulata, Stelechocarpus burahol, Potassium oxonate, Xanthine oxidase.
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