ANTIHYPERURICEMIC ACTIVITY OF FOUR PLANTS ANNONACEAE USING HYPERURICEMIC RATS MODEL AND ENZYME ASSAY

Authors

  • TITIK SUNARNI Setia Budi University
  • FRANSISKA LEVIANA
  • IRDA FIDRIANNY
  • MARIA IMMACULATA IWO
  • KOMAR RUSLAN WIRASUTISNA

Abstract

Objective: The aim of the research is to study the antihyperuricemic activity of Annona muricata L, Annona squamosa L, Annona reticulata L, and
Stelechocarpus burahol (Bl.) Hook. F. & Th. (Annonaceae).
Methods: The dried powdered leaves were extracted using ethanol by maceration method. The ethanolic extracts of four plants were administered
orally to potassium oxonate-induced rats. Serum samples were collected from rats at the 1
st
to 3
hr after drug administration. Enzyme assay was done
by xanthine oxidase (XO). The XO inhibitory activity was measured by UV-Vis spectrophotometry, and the degree of enzyme inhibition was determined
by measuring the absorbance of uric acid formation.
rd
Results: The result of this study revealed that all of the ethanolic extracts had a significant effect on hyperuricemic rats, and S. burahol extract
decreased uric acid level was equal to level normal control. However, the antihyperuricemic activity was not parallel with XO inhibitor activity which
showed weak activity with IC
50
of A. reticulata was 171.73 µg/ml, while IC
50
2
of A. muricata, A. squamosa, and S. burahol more than 200 µg/ml.
Conclusion: The ethanolic extract of S. burahol was more potential than three other extracts in decreasing in uric acid levels. The results scientifically
confirmed that S. burahol can be developed as potential antihyperuricemic agents.
Keywords: Antihyperuricemia, Annona muricata, Annona squamosa, Annona reticulata, Stelechocarpus burahol, Potassium oxonate, Xanthine oxidase.

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Author Biography

TITIK SUNARNI, Setia Budi University

Biology Pharmacy

References

Yu KH. Febuxostat: A novel non-purine selective inhibitor of xanthine

oxidase for the treatment of hyperuricemia in gout. Recent Pat Inflamm

Allergy Drug Discov 2007;1(1):69-75.

So A, Thorens B. Uric acid transport and disease. J Clin Invest

;120(6):1791-9.

Preitner F, Bonny O, Laverrière A, Rotman S, Firsov D, Da Costa A,

et al. Glut9 is a major regulator of urate homeostasis and its genetic

inactivation induces hyperuricosuria and urate nephropathy. Proc Natl

Acad Sci U S A 2009;106(36):15501-6.

Jin M, Yang F, Yang I, Yin Y, Luo JJ, Wang H, et al. Uric acid,

hyperuricemia and vascular diseases. Front Biosci (Landmark Ed)

;17:656-69.

Choi HK, Mount DB, Reginato AM; American College of Physicians;

American Physiological Society. Pathogenesis of gout. Ann Intern Med

;143(7):499-516.

Wang SY, Yang CW, Liao JW, Zhen WW, Chu FH, Chang ST. Essential

oil from leaves of Cinnamomum osmophloeum acts as a xanthine

oxidase inhibitor and reduces the serum uric acid levels in oxonateinduced

mice. Phytomedicine 2008;15(11):940-5.

Masuoka N, Nihei K, Masuoka T, Kuroda K, Sasaki K, Kubo I.

The inhibition of uric acid formation catalyzed by xanthine

oxidase properties of the alkyl caffeates and cardol. J Food Res

;1(3):257-62.

Pacher P, Nivorozhkin A, Szabó C. Therapeutic effects of xanthine

oxidase inhibitors: Renaissance half a century after the discovery of

allopurinol. Pharmacol Rev 2006;58(1):87-114.

Fagugli RM, Gentile G, Ferrara G, Brugnano R. Acute renal and

hepatic failure associated with allopurinol treatment. Clin Nephrol

;70(6):523-6.

de Sousa OV, Vieira GD, de Jesus RG. de Pinho J, Yamamoto CH,

Alves MS. Antinociceptive and anti-inflammatory activities of the

ethanol extract of Annona muricata L. leaves in animal models. Int J

Mol Sci 2010;11(5):2067-78.

Kumar AS, Venkatarathanamma V, Saibabu VN, Ram KS. Antipyretic

activity of Annona plants leaves on brewer’s yeast induced febrile rats.

Asian J Pharm Clin Res 2015;8(3):210-2.

Adewole SO, Ojewole JH. Protective effects of Annona muricata

Linn. (Annonaceae) leaf aqueous extract on serum lipid profiles and

oxidative stress in hepatocytes of Streptozotocin-treated diabetic rats.

Afr J Trad CAM 2009;6(1):30-41.

Liaw CC, Chang FR, Lin CY, Chou CJ, Chiu HF, Wu MJ, et al. New

cytotoxic monotetrahydrofuran annonaceous acetogenins from Annona

muricata. J Nat Prod 2002;65(4):470-5.

Sri-Wahjuni S, Putra-Manuaba IB, Rahayu-Artini NP, Wahyu-Dwijani S.

Uric acid inhibition activity of Annona muricata L. leave extract in

hyperuricemia induced wistar rat. Adv Pure Appl Chem 2012;2(1):8690.

Gupta RK, Kesari AN, Diwakar S, Tyagi A, Tandon V, Chandra R, et al.

In vivo evaluation of anti-oxidant and anti-lipidimic potential of Annona

squamosa aqueous extract in Type 2 diabetic models. J Ethnopharmacol

;118(1):21-5.

Soni VK, Yadav DK, Bano N, Dixit P, Pathak M, Maurya R, et al.

N-Methyl-6, 7-dimethoxyisoquinolone in Annona squamosa twigs is

the major immune modifier to elicit polarized Th1 immune response in

BALB/c mice. Fitoterapia 2012;83(1):110-6.

Tomar RS, Sisodia SS. Antidiabetic activity of Annona squamosa L. in

experimental induced rats. Int J Pharm Biol Arch 2012;3(6):1492-5.

Rajini R, Nisha JM. Potential bioactivity of Annona reticulata L. leaf

extract and its compound identification by GC-MS. Int J Sci Innov

Discov 2013;3(1):202-11.

Thang TD, Kuo PC, Huang GJ, Hung NH, Huang BS, Yang ML, et al.

Chemical constituents from the leaves of Annona reticulata and their

inhibitory effects on NO production. Molecules 2013;18(4):4477-86.

Sunarni T, Pramono S, Asmah R. Antioxidant free radical scavenging

of flavonoid from the leaves of Stelechocarpus burahol (Bl.) Hook f. &

Th. Indonesian J Pharm 2007;8(3):111-6.

Sutomo. Decrease of uric acid degree on broiller cock hyperuricaemia

by ether petroleum fraction of kepel leaves (Stelechocarpus burahol

Hook.). Sains dan Terapan Kimia 2008;2(1):14-22.

Purwantiningsih, Hakim AR, Purwantini I. Anti-hyperuricemic activity

of the kepel (Stelechocarpus burahol (Bl.) Hook. F. & Th.) leaves

extract and xanthine oxidase inhibitory study. Int J Pharm Pharm Sci

;2(2):122-7.

Liu X, Chen R, Shang Y, Jiao B, Huang C. Lithospermic acid as a novel

xanthine oxidase inhibitor has anti-inflammatory and hypouricemic

effects in rats. Chem Biol Interact 2008;176(2-3):137-42.

Abdullahi A, Hamzah RU, Jigam AA, Yahya A, Kabiru AY,

Muhammad H, et al.. Inhibitory activity of xanthine oxidase by

fractions Crateva adansonii. J Acute Dis 2012;1(2):126-9.

Hu QH, Zhu JX, Ji J, Wei LL, Miao MX, Ji H. Fructus gardenia extract

ameliorates oxonate-induced hyperuricemia with renal dysfunction in

mice by regulating organic ion transporters and mOIT3. Molecules

;18(8):8976-93.

McGill NW. Review: The epidemiology and treatment of gout.

Rheumatol Res Rev 2011;3:73-82.

Reinders MK, Jansen TL. Management of hyperuricemia in gout:

Focus on febuxostat. Clin Interv Aging 2010;5:7-18.

Nagao A, Seki M, Kobayashi H. Inhibition of xanthine oxidase by

flavonoids. Biosci Biotechnol Biochem 1999;63(10):1787-90.

Nguyen MT, Awale S, Tezuka Y, Shi L, Zaidi SF, Ueda JY, et al.

Hypouricemic effects of acacetin and 4,5-o-dicaffeoylquinic acid

methyl ester on serum uric acid levels in potassium oxonate-pretreated

rats. Biol Pharm Bull 2005;28(12):2231-4.

Mo SF, Zhou F, Lv YZ, Hu QH, Zhang DM, Kong LD. Hypouricemic

action of selected flavonoids in mice: Structure-activity relationships.

Biol Pharm Bull 2007;30(8):1551-6.

Purwantini P, Santoso D. Identification of standard parameters of kepel

leaves (Stelechocarpus burahol (Bl.) Hook. F. & Th.) and the extract as

raw material for anti-hyperuricemic medicaments. Asian J Pharm Clin

Res 2011;4(1):149-53.

Chavan MJ, Wakte PS, Shinde DB. Analgesic and anti-inflammatory

activity of caryophyllene oxide from Annona squamosa L. bark.

Phytomedicine 2010;17:149-51.

Reddy SK, Reddy CS, Ganapaty S. Analgesic and anti-inflammatory

activity of stem bark of Annona reticulata Linn. J Chem Pharm Sci

;4(3):100-4.

Cos P, Ying L, Calomme M, Hu JP, Cimanga K, Van Poel B, et al.

Structure-activity relationship and classification of flavonoids as

inhibitors of xanthine oxidase and superoxide scavengers. J Nat Prod

;61(1):71-6.

Chen CY, Huang CC, Tsai KC, Huang WJ, Huang WC, Hsu YC, et al.

Evaluation of the Antihyperuricemic Activity of Phytochemicals from

Davallia formosana by Enzyme Assay and Hyperuricemic Mice Model.

Evid Based Complement Alternat Med 2014;2014:873607.

Huang J, Wang S, Zhu M, Chen J, Zhu X. Effects of genistein, apigenin,

quercetin, rutin and astilbin on serum uric acid levels and xanthine

oxidase activities in normal and hyperuricemic mice. Food Chem

Toxicol 2011;49:1943-7.

Published

01-11-2015

How to Cite

SUNARNI, T., F. LEVIANA, I. FIDRIANNY, M. I. IWO, and K. R. WIRASUTISNA. “ANTIHYPERURICEMIC ACTIVITY OF FOUR PLANTS ANNONACEAE USING HYPERURICEMIC RATS MODEL AND ENZYME ASSAY”. Asian Journal of Pharmaceutical and Clinical Research, vol. 8, no. 6, Nov. 2015, pp. 250-3, https://journals.innovareacademics.in/index.php/ajpcr/article/view/8559.

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