• Effionora Anwar Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.
  • Putri Amalia Handayani Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.


Objective: The aim of this study was to prepare transfersome-loaded microspheres which had good characteristics and physicochemical stability to
increase bioavailability of the polyphenol component of green tea leaf extract in the body.
Methods: Transfersomes were prepared using a thin-layer hydration method. Green tea leaf extract transfersomes were formulated in the ratio of
95:5, 90:10, and 85:15 based on their phospholipid and Span 80 content.
Results: The most successful formula produced transfersomes of a spherical shape, 78.75 nm in size with a polydispersity index of 0.187, zeta
potential of −37.5 mV, and entrapment efficiency of 47.96±5.81%. Subsequently, the transfersome was loaded into a microsphere using the spray-dry
method. The microspheres had a non-spherical, wrinkled shape, their size was 2058.44 nm, their polydispersity index was 0.545, their entrapment
efficiency was 59.27±0.59%, their moisture content was 5.21%, and their swelling index was 289.36% after 4 h. The total cumulative amount of
Epigallocatechin-3-gallate after a dissolution test was 69.15±7.66%.
Conclusion: The physicochemical stability of transfersome-loaded microspheres was not significantly different from that of transfersome powder.

Keywords: Dissolution profile study, Epigallocatechin gallate, Green tea leaf extract, Microsphere, Transfersome.


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How to Cite
Anwar, E., & Handayani, P. A. (2018). FORMULATION AND DISSOLUTION PROFILE STUDY OF TRANSFERSOME-LOADED MICROSPHERES FROM GREEN TEA LEAF EXTRACT. International Journal of Applied Pharmaceutics, 10(1), 211-215.
Original Article(s)